FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_325_rr.352000000a96ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_325_rr.352000000a96ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634681
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT5400.069.851485
CCATACG3200.065.737635
ACGAGCG702.3269968E-762.17559
TAGTAAC15200.058.6985782
ACGCACT7150.055.798524
CACGAGC7150.055.798528
AGCACGC7500.053.1945951
GTAGTAA18700.050.039641
ACACGAG8400.048.358727
CACGCAC8900.045.641943
TACGTGC5000.044.973618
AACACGA9200.044.942076
CGCCGTT1557.421477E-1042.08793145
TAACAAG21650.041.211015
TAGACCG907.4613956E-540.298939
GCACGCA9550.040.2567332
AAACACG12200.039.2419135
GTAACAA23750.038.1779374
AGTAACA24550.036.638383
GCGGTGC4400.029.674679
Produced by FastQC (version 0.11.2)