FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_323_rr.352000000a96a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_323_rr.352000000a96a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800415
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA24950.070.941261
TAGTAAC24250.070.296832
CGCACTT5300.062.96485
GTAACAA30000.058.037124
ACGAGCG1600.054.4082879
AAGAGCG8100.052.840979
AGTAACA34600.050.32123
AAACACG15350.048.6793445
TAACAAG36350.047.1002735
ACGCACT7550.045.161334
CGACGAA1801.8189894E-1244.294468145
AACACGA14350.043.4772836
ACACGAG16400.038.0415657
ACAAGAG48650.034.7437637
CACGAGC18050.034.5640838
GTAGAAA36500.034.1833951
CTGCGCG850.00274593234.10911145
GCACGCA9700.033.6527372
AGCACGC9550.033.421731
CAAGAGC49350.033.3689468
Produced by FastQC (version 0.11.2)