FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_322_rr.352000000a9687.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_322_rr.352000000a9687.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023227
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGTGTCTGAGGAAGCCCAGATATTGGACACACTAGACAAATACTTT24980.12346612614402636No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG21730.10740267898757777No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT20590.10176811598500812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA36700.0100.211881
TAGTAAC37950.095.764212
GTAACAA42300.085.918244
CGCACTT8550.083.147285
AGTAACA50450.072.468063
TAACAAG51000.070.692655
AAGAGCG11200.059.5878759
ACGCACT12600.056.9970934
GCACGCA12750.055.1872632
AGCACGC12850.054.757791
ACAAGAG67850.053.2436147
CAAGAGC67050.051.9314588
AAACACG19300.051.8692325
AACAAGA80850.044.7722136
AACACGA20650.043.560186
ACGAGCG2400.042.3160259
CTCGCTC8500.041.8171543
CACGCAC17600.041.6280443
TCTCGCT8550.040.7241862
TCGCTCT8600.040.4884224
Produced by FastQC (version 0.11.2)