FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_320_rr.352000000a966d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_320_rr.352000000a966d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497405
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA28750.086.052324
TAGTAAC29550.082.495052
CGCACTT5100.078.241755
GTAGTAA33750.073.731291
TAACAAG36350.068.4598165
CGGTGCA3600.064.490172
AGTAACA39550.062.1869543
AGCACGC6600.059.3582841
ACGAGCG753.7458085E-758.037279
ACAAGAG43700.057.4434787
ACGCACT7200.056.42894
AAACACG13500.056.42895
GCGGTGC7750.056.166981
TCGTAAC400.006034512554.413582
GCACGCA7250.053.037612
TCTCGCT3900.052.0882152
CTCGCTC3800.049.640463
AACACGA13800.049.4190986
GTCGTAA450.00961435748.3660131
CTGCGCG901.3267054E-648.31913145
Produced by FastQC (version 0.11.2)