FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_318_rr.352000000a9644.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_318_rr.352000000a9644.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215823
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT24720.11156125737479933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT9000.082.2215965
GTAGTAA27400.069.368031
ACGCACT12600.058.729714
GTAACAA31400.058.4546554
AGCACGC13550.055.680441
AACACGA14300.054.7918476
GCACGCA13500.054.2745482
TAGTAAC34550.053.7527282
AAACACG19300.045.8596535
ACACGAG17550.045.4719737
ACGAGCC6750.045.141279
AGTAACA41500.044.927613
TAACAAG42950.042.5662735
CACGAGC19800.040.6711128
AAGAGCG12700.039.4160969
ACGAGCG1209.525353E-636.274239
GTAGAAA45150.035.991411
ACGAGCT8000.035.367389
CACGCAC21650.033.5096863
GAAACAC28700.031.8505484
Produced by FastQC (version 0.11.2)