FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_316_rr.352000000a962a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_316_rr.352000000a962a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574238
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAA45960.17853827035417857No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT38180.1483157345979665No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA37750.14664533737750743No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT37260.1447418614751239No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC35010.1360014109029546No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT29530.11471355795384887No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG28640.11125622417196857No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA27780.10791542973105052No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT27690.10756581170816373No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA27630.10733273302623923No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT27480.10675003632142793No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT25980.10092306927331507No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA28050.0114.834214
GAAACAC13600.096.551924
CGGTGCA4600.096.2002642
GGTGCAT4750.093.165993
AAACACG14800.089.703745
TAGAAAC14900.089.585112
GAGCAGA4150.089.151031
TAGTAAC40750.079.576382
CACGCAC651.5570549E-978.1278463
TAACAAG43800.074.037995
ACAAGAG44200.072.710017
AACACGA17500.071.7181856
GGCACCC651.4018406E-766.90293145
TTCGTCC1009.094947E-1265.23036145
ACACGAG19600.063.662717
TTCGGAA1300.061.327686145
GCACGCA851.3000317E-859.7425082
TATCCGA2950.059.0192262
GCTGCCC2750.057.98254145
GCGGAGC5900.057.7896581
Produced by FastQC (version 0.11.2)