FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_310_rr.352000000a9600.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_310_rr.352000000a9600.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358753
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA27950.060.7311631
CGCACTT9450.059.876835
TAGTAAC32750.050.05922
ACGCACT12250.048.559454
AAGAGCG14450.045.1835069
GTAACAA37050.044.8376964
AACACGA13600.041.605596
TAACAAG42150.039.9288145
AGTAACA43400.038.610823
AGCACGC15350.037.333291
AAGCGGT5500.036.9318127
AAACACG17850.036.1699455
ACGAGCG2052.910383E-1035.387629
GCACGCA17300.033.964542
ACACGAG17100.033.9390077
GTAGAAA42000.033.8520051
CACGAGC17650.032.4703988
TAGTAGT12150.031.0472474
ATTAGTA23050.028.953061
ACGAGCC5600.028.4996749
Produced by FastQC (version 0.11.2)