FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_306_rr.352000000a95e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_306_rr.352000000a95e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597833
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA43190.16625395088906794No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC41370.15924811179163556No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT40880.15736192434232685No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA40550.15609163483564956No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT38130.1467761784533494No Hit
CTATAGAACATGCTGGTGAATTCAAACATTCCAGTTTCGTTGCAGTAACT35370.13615193894295746No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG35070.13499713030052354No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG33630.1294540488168408No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC32310.12437289079013161No Hit
ATTGCTACTTCTTTCATTATTTTCTGGTATGTTAATCCATCCCCTGTGGC30480.11732855807128481No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT30390.11698211547855462No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT29360.11301727247286489No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG28310.1089754422243462No Hit
GTATTCAGGGTCCATTAGATTGTACTTCAACACTATTTCTGGGATATGCA28070.1080515953103991No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG28060.10801310168898463No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA27680.10655034407523502No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT27240.10485662473299863No Hit
GTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA26360.10146918604852583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA6200.0114.664982
GAAACAC15200.0102.135084
AAACACG15850.097.946585
GGTGCAT7300.097.3885653
AGCCACC2550.093.79978145
GCTCGGA259.3662844E-487.051772
AACACGA17750.081.740336
AGCACGC1500.077.377871
CGCACTT1600.077.078585
GCACGCA1550.074.8832552
ACACGAG19800.073.278737
ATTCGGG903.6379788E-1272.48164145
AACGGCC502.3107532E-672.48164145
GTAACAA50100.071.530954
GCGGAGC8850.071.3122251
CACGCAC1650.070.346233
GCGGTGC13750.069.640081
GCTGCCC1750.066.268936145
ACGCACT1800.064.484044
ACGTGCA1850.062.7400172
Produced by FastQC (version 0.11.2)