FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_302_rr.352000000a95cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_302_rr.352000000a95cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517919
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1350.075.166855145
GTAGTAA19850.057.01421
TAGTAAC20400.053.3450782
CGCACTT4600.048.8935365
GTAACAA24850.042.9179084
TAACAAG26000.041.0196155
AACACGA8400.037.139566
AGCACGC5350.036.612461
ACGCACT6350.036.5614854
AAGAGCG8750.034.8248189
AGTAACA32450.033.9830323
CACGAGC9000.033.857468
CGCCGTT1551.4684629E-632.73395145
ACACGAG9850.031.6723167
CGACGAA4500.030.603647145
AAGCGGT3600.030.2298777
GGTTCGT2252.7996066E-829.020688
GAAGCGG6000.027.8114856
GTAGCTA5300.027.3780028
ATGGTCG1606.797354E-527.2068885
Produced by FastQC (version 0.11.2)