FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_297_rr.352000000a95a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_297_rr.352000000a95a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141451
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC47430.22148533867924133No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT43790.20448751804267293No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA42850.20009797095520745No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT38450.17955115480111383No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT35160.1641877399949847No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA30010.1401386256328069No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG28270.1320132937900517No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT26950.1258492489438236No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT26550.12398135656617873No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG26460.12356108078120864No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC25610.11959180947871326No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA25170.1175371278633039No Hit
CCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT24180.11291409422863283No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT23340.10899152023557859No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT23150.10810427135619727No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT22810.10651656283519911No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG22380.10450857852923089No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC22120.10329444848376171No Hit
ATTGCTACTTCTTTCATTATTTTCTGGTATGTTAATCCATCCCCTGTGGC21720.10142655610611684No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG21590.10081949108338224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAC11700.0102.316374
CGGTGCA3650.0101.368742
AGCACGC1200.096.7264251
AAACACG12400.095.370265
ACACGTG1100.092.334097
CGCACTT1450.080.056955
TGACGCC1650.079.06395145
ACGCACT1500.077.388384
GCACGCA1500.077.3847662
CGGAGCG1350.075.235182
GGTGCAT4950.073.2844543
AACACGA14850.073.284456
CACAGCG300.001930587572.5516053
CGCACCC406.676186E-572.475296145
GGCACCC502.3118173E-672.47529145
GCGGAGC6550.070.883491
GTAACAA37500.069.649544
AACACGT1550.065.530496
ACACGAG16700.065.163077
GGAGCGT1450.065.046273
Produced by FastQC (version 0.11.2)