FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_291_rr.352000000a9588.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_291_rr.352000000a9588.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2004061
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCC4100.058.341145145
ACACGAG8250.056.2697147
GCGTCGC400.00605690554.36334145
GTAGTAA17200.053.9783131
CACGAGC9050.051.2955938
TAGTAAC17550.050.4220542
AAACACG10750.049.2564435
AACACGA9950.047.384796
CGGTGCG800.002032257436.2675867
ACGAGCA5900.035.652889
AGTAACA26800.033.0197453
GCCGACC4400.032.947475145
TAACAAG27550.032.647415
GTAACAA27800.031.8319844
GACGCTT3300.030.750982145
GTATACG1504.387096E-529.0133441
AAGCGGT3153.45608E-1127.6324484
TTAAGCG1050.007692037627.6324463
TAGAAAC37850.026.828722
CAAGAGC33800.026.8251388
Produced by FastQC (version 0.11.2)