FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_286_rr.352000000a956e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_286_rr.352000000a956e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474295
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCC2750.050.07991145
GTAGTAA14850.048.8506241
AACACGA5450.047.920066
ACGAGCC2300.047.3075569
TAGTAAC15600.046.0370182
CGCACTT2700.045.6768885
TTATCGC803.7231526E-545.3379488
GCCGACC2100.044.871098145
ACACGAG6000.043.524437
AAACACG9050.043.2869075
TAACAAG16300.042.726275
GTAACAA17450.039.4947624
CGGAGCG800.00203132136.270367
ACGAGCG1102.4291717E-432.971939
ATCGACC900.003632689432.215145145
CACGAGC8200.031.8471418
ATTATCG1204.045639E-430.2252967
TATCGCC1204.0464444E-430.224279
AAGAGCG6200.029.2492969
GTAGAAA25600.029.1872961
Produced by FastQC (version 0.11.2)