FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_285_rr.352000000a9552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_285_rr.352000000a9552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518999
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT56020.22238992552200296No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT45770.18169915907072612No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT41640.1653037575640165No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT39550.1570068110388293No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC38990.15478370574978395No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC38150.15144904781621588No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT36460.14474003364034682No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA32360.12846372705983608No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT31630.1255657505223305No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT31540.12520846574373393No Hit
GTGGAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTT30300.12028587546084775No Hit
GCAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATA30150.11969040082985345No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT30090.11945221097745572No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC29710.11794367524560351No Hit
CCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT29660.11774518370193876No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT29250.11611755304388768No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC28980.11504569870809794No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG28980.11504569870809794No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT27710.1100040134990129No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC27480.10909095239815499No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA35000.0107.58414
CGGTGCA5600.0102.347962
AGCACGC2200.095.632791
GAAACAC12850.093.160024
GAGCAGA5000.092.862731
AAACACG13000.092.08515
ACGCACT2300.091.478484
TTCGGAA2100.086.28549145
TAACAAG44150.085.6161655
CGCACCC554.4055923E-879.068886145
TAGAAAC16900.078.1312562
CGCACTT2700.077.926115
AACACGA15150.074.70696
GCGGAGC7800.074.409241
GCACGCA2900.072.550462
TAGTAAC53350.071.802512
CACGCAC2950.071.3207863
CGGAGCA6100.070.171752
CCCTCGA1150.069.394711
GTAGAAA19350.068.9871751
Produced by FastQC (version 0.11.2)