FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_284_rr.352000000a9538.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_284_rr.352000000a9538.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219745
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT34840.15695496554784447No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC28340.12767232272175408No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG26510.11942813251071631No Hit
CTATAGAACATGCTGGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT26490.11933803207125142No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC25510.11492311053747165No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA25450.11465280921907697No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT23080.1039759071424871No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG22470.10122784373880783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG9150.067.391287
AGCACGC2400.066.491841
CGCACTT2250.064.4856645
AACACGA9850.062.6034666
TCGCGCC350.00357047662.127914145
CACGAGC10650.056.537218
ACGAGCC4050.055.5268179
AAACACG13150.052.961615
AGAAGCG7050.050.4223025
AAGCGGT5400.048.3631557
CGGAGCG752.5433277E-548.359882
GAAACAC14050.048.0200164
TGACGCC1551.4551915E-1146.76295145
AGCGGTG5800.045.0277638
ACGCACT3600.044.3338934
GCCGACC1151.4163015E-744.119827145
GAAGCGG8400.042.3187146
GCGGTGC6450.040.489179
TAGACCG800.002030764736.2731865
AATACGC800.002030764736.2731865
Produced by FastQC (version 0.11.2)