FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_282_rr.352000000a94f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_282_rr.352000000a94f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498375
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT74650.21338478579340409No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA64480.18431414585343195No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT56550.1616464787222639No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT53870.15398577911173045No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT50410.14409547289813127No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA48620.13897881159109587No Hit
GTATGCAGAAACCAACCTGCTGCAACGGCACTGAATACAACAATAACCTC46970.13426233608461072No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT43300.12377175117018616No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG42260.12079894236609855No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC42130.12042734126558759No Hit
ATTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTGCATA42040.12017007896523385No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA40650.11619680565977061No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC39280.11228070175438595No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACTGGCAAGA38260.10936506235037698No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT37720.10782148854825455No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA36140.10330510594204452No Hit
GTATTAGTAGTTGAAAACTTGGCTAAAAATATATGTGAAAATCTAGAACA35980.10284775074141565No Hit
TTCTAATGGTATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC35870.10253331904098331No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA35320.1009611605388216No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA35070.10024654303783899No Hit
ATCTATCACAGTCTTGTTTGCAATTTCTACAGCTAGCTCCAGTGGGTTTG34990.10001786543752456No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG100.0070973365144.96088145
GAGCAGA4600.0116.699151
CGTGCCC458.54925E-11112.747345145
CCTACCC259.398792E-486.97653145
ACACGTG1350.080.604187
CCCGAAA451.235514E-680.53382145
CACGAGC10550.080.451378
AACACGA10650.079.69716
ACACGAG10800.079.260787
GCCGACC2800.077.657616145
AAACACG12450.076.3322755
GCTGCCC3300.074.67681145
GAAACAC12900.073.6695254
ACGAGCC5450.073.20939
GCCGCCC903.6379788E-1272.48044145
TAGAAAC14150.070.236712
AACACGT1550.070.204646
CTTAGGG5500.068.52696145
TCTGACC4100.067.176994145
CACGTGC1650.065.948878
Produced by FastQC (version 0.11.2)