FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_280_rr.352000000a94d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_280_rr.352000000a94d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3351980
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC53390.15927899331141593No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG49570.14788274393045306No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT47890.1428707808519144No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC45960.13711298993430748No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA44930.1340401792373463No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC42100.12559740809909367No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATTCTTTTAC40340.12034678011205319No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG38850.11590164619120638No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA38600.11515581835213815No Hit
ATATTTGCTTTCTCCCCTGGTTTTAGCTTTTCAAGTTCTTCAATGGTTTT37300.11127751358898322No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG36950.11023335461428768No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT36860.10996485659222312No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT36420.10865219959546298No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT36020.10745887505295379No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC35510.10593738626125454No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT35130.10480372794587081No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT34770.10372973585761251No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA34110.10176075036247233No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA33950.10128342054546864No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA33920.10119392120478046No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG14700.087.847065
CGGTGCA5550.083.6562962
TGACGCC1600.077.01075145
AACACGA15700.076.706666
GAAACAC16650.076.6872254
ACACGAG16350.073.654957
CACGAGC18050.066.315028
ACGAGCC8550.065.332579
GCGGAGC7250.060.036921
AGAAGCG9200.053.622335
GCCGACC1900.053.406837145
GTAACAA53550.053.3778884
GGTGCAT9250.049.4102363
ATTCGGG1203.6343408E-948.320465145
GAGCAGA15150.047.8842281
GCGGTGC15900.044.713031
GTCGCCC2600.044.60351145
AAGCGGT9300.043.6834267
TAACAAG66100.043.682375
TAGTAAC69100.043.1494642
Produced by FastQC (version 0.11.2)