FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_279_rr.352000000a94bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_279_rr.352000000a94bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3010207
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT59120.19639845366116018No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT52280.1736757638262086No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA49080.16304526565781025No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC44690.1484615509830387No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC43400.1441761314089031No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT40020.13294766771853234No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG37700.12524055654644348No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG37130.12334699906019753No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG35570.11816463120310332No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATTCTTTTAC34570.1148426005254788No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA34260.11381277101541522No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT33220.11035785911068575No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA33180.11022497788358077No Hit
ATATTTGCTTTCTCCCCTGGTTTTAGCTTTTCAAGTTCTTCAATGGTTTT31780.10557413493490647No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT31560.10484328818582908No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT30990.10294973069958312No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA30300.1006575295320222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG12300.096.1371845
GAAACAC14300.082.691424
CGGTGCA5300.075.277782
AACACGA14500.075.04686
GCACGCA1650.074.7385942
AGCACGC1700.072.540411
GCGGAGC6000.071.3314061
AGCCACC3850.069.65386145
ACACGAG15850.068.655947
CACGCAC1850.066.659863
GCCGACC1400.062.123722145
GCGGGCC350.003571554362.123722145
CGATACC350.003571554362.123722145
CACGAGC17850.061.3698238
GGTGCAT6700.060.6317983
ACGCACT2000.058.036194
GCTGCCC2500.057.98214145
ACACGTG1650.057.1578067
CGCACTT2050.056.620675
ACCGCCC805.890197E-754.358257145
Produced by FastQC (version 0.11.2)