FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_274_rr.352000000a9496.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_274_rr.352000000a9496.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771271
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT42620.15379224911601933No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA34870.1258267415925761No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG32610.11767163875348172No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC31170.11247546703299677No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC30870.11139293125789576No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC30340.1094804513885506No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT29840.10767622509671555No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA28760.10377909630635185No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG27830.10042323540353866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA26850.084.028474
AAACACG12250.074.618215
GAAACAC12400.073.715574
CGGTGCA4550.073.341362
CAGGATT5250.073.236395
AACACGT2150.070.8596656
GAGCAGA6800.069.3399351
GCACAGG5750.068.128442
GCGGAGC6100.066.5943151
TAGCGAA451.1906361E-464.48372
CGGAGCA4650.063.963682
AACACGA12100.062.953836
TAGAAAC17100.061.514072
ACACGTG2600.061.386867
ACACGAG12600.059.8809787
GGAGCAT5150.056.3460043
CGCACTT651.0900954E-555.804225
GAGCATC7300.055.651334
ACGTGCA2100.055.2737469
CACAGGA7100.055.1754383
Produced by FastQC (version 0.11.2)