FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_264_rr.352000000a947c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_264_rr.352000000a947c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856787
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT26900.14487391391688975No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT24240.13054809194592595No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC23340.12570100932417128No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT22480.12106935259671679No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG20770.11185989561538293No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG20380.10975949314595589No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT20310.10938249783093053No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC19690.10604339646927731No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA19360.10426613284130058No Hit
ATTGCTACTTCTTTCATTATTTTCTGGTATGTTAATCCATCCCCTGTGGC19160.10318900336979955No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG19040.10254272568689894No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT18620.10028075379674675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA4550.0102.034432
CGCACTT2100.086.361935
ATTCGGG1100.085.65988145
GTAACAA31750.083.168584
AAACACG9600.081.612025
GAAACAC9650.081.189164
GCGGTGC8450.071.252411
GGTGCAT6800.067.20993
TAGTAAC40550.065.83172
AGCACGC2600.064.17011
AACACGA11650.064.137636
TAACAAG42300.062.5970845
ACGCACT2900.062.5379454
GCACGCA2900.060.0331882
GTAGTAA49600.055.282581
AGTAACA49200.054.9978073
ACACGAG13600.054.939947
ACAAGAG50550.052.2360277
GCGGAGC7200.049.367571
TCTCGGG3400.049.03156145
Produced by FastQC (version 0.11.2)