FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_249_rr.352000000a9453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_249_rr.352000000a9453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806413
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT39540.14089159364640913No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT34310.1222557050583788No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT33700.12008211193434465No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC32220.11480847615799956No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT31830.11341880186558428No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC31670.11284867907895238No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT31240.11131647408987913No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC30890.11006933049412186No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA30090.10721871656096232No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC29040.10347728577369047No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG28330.1009473659080114No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC28300.10084046788551791No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA31950.0103.539084
GAAACAC13350.095.6404654
GAGCAGA5400.095.3770451
AAACACG14000.091.71825
CGGTGCA5500.088.368842
TTCGGAA1650.087.85391145
TAGAAAC16650.081.908822
GCGGAGC9350.079.1348651
GAGCATC10500.078.072744
AACACGA15600.077.660856
ACACGAG16050.075.028777
TAACAAG44350.074.9173055
CGGAGCA7950.073.9102862
GCCGACC903.6379788E-1272.479485145
GGAGCAT8400.070.8169253
AGCATCT13600.067.211245
TAGTAAC51250.065.110492
CACGAGC19200.062.719368
ACAAGAG53000.062.414257
CGCCCGC350.003571176462.125267145
Produced by FastQC (version 0.11.2)