FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_137_rr.352000000a9439.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_137_rr.352000000a9439.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834984
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA57540.2029641084394127No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT43940.15499205639255811No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT41880.14772570145016692No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA41000.14462162749419397No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC33430.11791953675929034No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG33340.11760207465015676No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA32810.11573257556303669No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT32690.11530929275085856No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAACCTCATTTAGGTTGAA32080.11315760512228641No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA31930.11262850160706375No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG31870.11241686020097467No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC31620.1115350210089369No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC31120.10977134262486138No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC29470.10395120395741211No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT28950.10211697843797356No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC28850.10176424276115845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT2850.071.273295
ACGCACT3100.067.865644
AAACACG11100.064.7032245
AGCACGC2850.063.633491
AACACGA11400.058.5459186
ACGAGCC5800.057.535499
ACACGAG11500.057.405997
CACGAGC11750.055.5671778
GCACGCA3300.054.957172
GAAACAC13850.051.332214
AGAAGCG6250.048.7509355
GCCGACC1800.048.32047145
TAACAAG44800.046.4748045
TGACGCC2350.046.26428145
ACGAGCT1602.0008883E-1145.3404739
GTAACAA49100.042.404714
TAGTAAC50350.042.0708922
AAGCGGT5450.041.2647137
GAAGCGG7750.039.3152736
CGGTGCA4450.039.124572
Produced by FastQC (version 0.11.2)