FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_100_rr.352000000a941f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_100_rr.352000000a941f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390720
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC42550.0105.706332
GTAGTAA43050.0105.4874951
CGCACTT15100.095.6119845
GTAACAA47900.094.057244
TAACAAG55450.081.5122155
AGCACGC19850.068.340931
ACGCACT22600.065.487434
AGTAACA69200.065.4178853
GCACGCA23400.058.2842032
ACAAGAG78250.057.296917
AAGAGCG12000.056.2249159
CAAGAGC82600.052.347188
GTCTCGC10100.050.277821
AACACGA26850.049.173021
AACAAGA92300.048.8119436
TCGCTCT10550.046.074284
TCTCGCT10750.045.8890762
CTCGCTC10700.045.426473
TCTGTCG11350.042.8258558
GTAGAAA40400.039.324441
Produced by FastQC (version 0.11.2)