FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_zk-hu-0163_rr.351000000a9ae3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_zk-hu-0163_rr.351000000a9ae3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970585
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA63810.3238124719309241No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC53880.2734213444231028No Hit
GTATATAACACTTGTTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC39310.1994839096004486No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC34590.1755316314698427No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC34000.17253759670351698No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT29580.15010770913205979No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC26820.13610171598789192No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC25970.131788276070304No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC25440.129098719415808No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC24560.1246330404423052No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC24530.12448080138639034No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG23850.12103004945232No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT23700.12026885417274566No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC23160.11752855116627804No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA22510.11423003828812256No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAA22470.11402705288023607No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG21200.10758226617983999No Hit
ATAATATACAGATTATAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA21010.10661808549237917No Hit
GTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGA20360.1033195726142237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTC100.0070904084145.006654
TATCTAA301.5994823E-596.67112
CGAGTTT3150.087.4643254
TCGAGTT3150.087.4643253
CAACAGA750.087.003992
GTTCGAG3200.086.1020661
TTCGAGT3300.083.488682
ATGCCTT300.001935280372.5071
CCTGGAT300.001935669972.503332
AACAGAT1009.094947E-1265.252993
GTATCTA451.1935743E-464.450671
GGCTTCT8250.063.2788431
CAGGGAT350.00356565362.1488571
GCGTCAG350.00356565362.1488571
TACTTCA350.00356708762.142558
CAACTCC702.3357825E-762.14255145
GTTTGAA4600.059.8910067
AGTTTGA4650.059.2500346
ACAGGTC502.0097653E-458.0056041
AGTGCAG502.0102697E-458.002662
Produced by FastQC (version 0.11.2)