FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_vww_10e3_p2_rr.351000000a9a29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_vww_10e3_p2_rr.351000000a9a29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976111
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT63310.3203767399705786No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC27350.13840315650284826No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA26150.13233062312795182No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC25910.13111611645297253No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGCATCGCATGTAT24160.12226033861458187No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT22720.11497329856470613No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC22220.11244307632516594No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT21930.11097554742623263No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT21470.10864774296585565No Hit
GGATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAGGCCAAATG21250.10753444518045799No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG20570.10409334293468332No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT20140.10191735180867877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT5150.0112.6208655
GTAACAA24700.0107.428684
AGCACGC5950.098.699551
CGGTGCA7400.096.992822
TAGTAAC27650.095.704832
GCACGCA6350.092.4799042
GAAACAC11150.076.07594
CGAGCTA1250.075.399675
TAACAAG35400.074.3428965
AAACACG11500.073.7605445
ACAAGAG36750.071.414667
ACGCACT8450.070.354724
CACGCAC9000.066.055273
AGCATCT7600.063.9141925
AGTAACA42400.063.437223
GTGCGAT350.003567079862.142594
GAGCATC7450.060.335314
TAGAAAC17250.058.8403242
AACACGA14150.055.335456
CTCGCTC2350.052.4465833
Produced by FastQC (version 0.11.2)