FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_vww_10e3_p1_rr.351000000a98f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_vww_10e3_p1_rr.351000000a98f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658300
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA73310.4420792377736236No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT32170.19399384912259543No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG27540.16607368992341556No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC23320.14062594222999455No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT22210.13393234034854973No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA21930.13224386419827533No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT21460.12940963637460048No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT21370.1288669118977266No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC20030.12078634746427064No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT19640.11843454139781703No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT19270.11620334077066877No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT19040.11481637821865766No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA18260.11011276608575048No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC17700.10673581378520172No Hit
GCAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATA17480.10540915395284328No Hit
ACATTGTCTCTGGAGACCATATAAACAACAAAGATAGCTATCATCAGTGT17390.10486642947596936No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC17380.1048061267563167No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTT100.0070901383145.007815
CACAGCG100.0070901383145.007813
TACCGAG100.0070901383145.007812
AACGGCC100.0070914165144.99905145
AGGATTG9150.0132.329535
CACGCAC450.0128.895833
GCACGCA450.0128.895832
GAGCAGA2350.0117.250961
AGCACGC501.8189894E-12116.016751
CGGTGCA2550.0110.888332
ACTTGGT203.866146E-4108.752588
TTCGGAA203.8666098E-4108.7493145
ACGCACT553.6379788E-12105.460224
GAAACAC9300.0101.349554
GTAACAA24000.097.578174
CGCACTT607.2759576E-1296.6718755
TAGTAGA1900.095.399883
AACGGGG259.388086E-486.999435145
ACGTGCA1200.084.587892
GGATTGA14450.083.793446
Produced by FastQC (version 0.11.2)