FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_rt-pcr_neg_ctrl_rr.351000000a9900.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_rt-pcr_neg_ctrl_rr.351000000a9900.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12059
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAAACACGAGCAACGGGCACGTACTCTACCGCACCTGCAGATGTTTT260.2156066008790115No Hit
TCCTTAAAGCGTTCCATTGAATCAGGCACAGAAGCACAGCGCGAAGCTGT230.1907289161622025No Hit
GCAGATACCGCGCAAAAACATCTGACTCAACCGATTATCACAGAAGCGAA200.1658512314453935No Hit
GTATAGCAGTGGTCGGCATGATCGCATGATTGAACTGATTCGTCAGGGTA200.1658512314453935No Hit
GGCCTGAACGCAGCGCCTCCGTCACGGAGTGCTTACCGGCGATCCATTCT170.14097354672858448No Hit
TCTATATTCCAAAACGTCTGCGTTTTTGGGAATACTGCCGGACTTGGCAT170.14097354672858448No Hit
GTATATGAGGTAGCCGTACGACAGTATCTGTTATCTAAAGCCAACATGCG160.1326809851563148No Hit
GTACTCTACCGCACCTGCAGATGTTTTGGATACGGTCACTGTAACTGCAG160.1326809851563148No Hit
CTGTAAGGTACGCTGCTCATGGCGTAGTAAATTACAAGGCGTTATAGAGA150.12438842358404512No Hit
CTCTATAACGCCTTGTAATTTACTACGCCATGAGCAGCGTACCTTACAGA150.12438842358404512No Hit
CTCCAAAGCTGTGGCATGTCTATATTCCAAAACGTCTGCGTTTTTGGGAA140.11609586201177544No Hit
TTTCTGAACTCTGCAGGAAAAATAGCAGCGTTCGCTCGTTTAGAAGGATT140.11609586201177544No Hit
GTTATAGAGAGGATCGAATAAAGATGGAAGAAGAATGGATCGCCGGTAAG140.11609586201177544No Hit
ATATTGGGGATGCGGTATATGAGGTAGCCGTACGACAGTATCTGTTATCT130.10780330043950576No Hit
GTAGTGCGACAGGCTTGGTATCGGCGAAGGCACAGAGCCGTATTCTCACA130.10780330043950576No Hit
CTTCAATCTCATCCAGCAAAATCAAAAATGGATGTTCATTTTTTGCAGCC130.10780330043950576No Hit
GTGATAATCGGTTGAGTCAGATGTTTTTGCGCGGTATCTGCAATCCATAT130.10780330043950576No Hit
CTGCTATACAGGTAACCGATCAGACACTCCAAAGCTGTGGCATGTCTATA130.10780330043950576No Hit
GCTTTGGAGTGTCTGATCGGTTACCTGTATAGCAGTGGTCGGCATGATCG130.10780330043950576No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCG100.007004778145.07
AATCTCG100.007004778145.06
GTGTAGC100.007004778145.01
CGGACAT100.007004778145.07
GCGGACA100.007004778145.06
GACGCCG100.007004778145.07
AGCGGAC100.007004778145.05
GCAGAAA100.007004778145.01
CTACGAC100.007004778145.03
TAGAGAG100.007004778145.05
ACGACGC100.007004778145.05
GCACTGG100.007004778145.06
AGGTACG100.007004778145.0145
GTTATAG100.007004778145.01
TATAGAG100.007004778145.03
GACTACG100.007004778145.01
TTCTGCA203.7730287E-4108.753
TGCAAGC259.161697E-487.06
CTGCAAG300.001889501572.55
AAGCTCC300.001889501572.59
Produced by FastQC (version 0.11.2)