FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_prvabc59b_rr.351000000a9b5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_prvabc59b_rr.351000000a9b5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548517
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA80570.3161446441204826No Hit
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC69850.27408096551837796No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC61660.2419446289744192No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC53840.2112601171583317No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC49470.19411288996698864No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT49210.19309268880686295No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT42630.16727375175445172No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC38780.15216692688336No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT34640.1359221853336666No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC33460.13129204160694238No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC33150.1300756479160233No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC31500.12360129439984116No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA31190.12238490070892209No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC30540.11983439780860791No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC29730.11665607880975484No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG29190.11453719947718616No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC28550.11202593508303064No Hit
ATAATATACAGATTATAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA28370.11131964197217441No Hit
GCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGT26440.10374661028354921No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC26060.10225554704951939No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA26050.1022163085433607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCC259.3886E-486.999596145
GTTCGAG3400.081.03541
GTCATAA9450.078.259751
TCGAGTT3350.077.913153
CGAGTTT3350.077.911614
TTCGAGT3350.077.911612
GACAGGG300.001935520272.5053561
TTCTCGA702.8139766E-972.5053561
GTAATAG300.001935821672.502513
TAATAGG300.001935972372.501094
TACATTA502.3070625E-672.501092
TACACTT300.001935972372.501095
AGGTATA406.66467E-572.501082
CTCGACC1450.070.001052
ACAGTTC1200.066.460643
ACAGGTC554.061625E-665.913961
TCTCGAC1550.065.488711
CGACCCT1550.065.4848564
TCATAAT11900.062.7530442
ATAATAC12100.061.1166234
Produced by FastQC (version 0.11.2)