FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pr8_c6_rr.351000000a9852.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pr8_c6_rr.351000000a9852.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320253
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG158370.6825548765587202No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA94310.40646429505747866No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG80430.34664323244060025No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA52510.22631152723431455No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC49660.21402838397364424No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA45250.19502183598081763No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT44850.19329788604949547No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG44570.19209112109756996No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG44090.19002238117998338No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA42020.18110094028539128No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT41950.1807992490474099No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT41900.18058375530599463No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT40680.1753257080154621No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT39310.16942117950068378No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT37630.16218058978913075No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT36750.15838789994022204No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC35620.153517741384237No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA34640.1492940640524977No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG33310.1435619305308516No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT32460.13989853692679205No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC31130.1341664034051459No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC29920.12895145486289641No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA28150.12132297641679593No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA27760.11964212523375684No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT27020.11645281786081087No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA27020.11645281786081087No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT26660.11490126292262094No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT26080.11240153552220383No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA25890.11158265930482582No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA25210.10865194442157816No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT24880.10722968572823739No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT24730.10658320450399159No Hit
ATATTATTGTGTCTCCGGGTTTTAGCAAGGTCCAGTAATAGTTCATCCTC24450.10537643955206609No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT24170.10416967460014058No Hit
GGCTACGGCAGGTCCATACACACAGGCAGGCAGGCAGGACTTGTGAGCAA24140.10404037835529142No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT23710.10218713217912012No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGCTCGCTGCCTTTTCGTC23490.10123895971689294No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTT100.007091282145.001226
GAGCGAA10900.0137.699481
GCAAAAG72550.0104.231253
AGCAGGC4000.0103.311158
TATTAGT750.096.67791
GCAGGGT49550.089.398439
GCAGGGG17500.087.8274159
AGCAAAA88850.085.2726752
ATTAGTA2050.081.350921
AGCAGGG74100.080.717578
AGCGAAA18850.079.233152
AAGCAGG118950.075.8207867
CAAAAGC101100.075.1539464
GAGCAAA102700.074.202881
TACTAAT1400.072.499057
CTACTAA1450.070.0005956
CGTACCT754.8585207E-967.667245
TTAGTAG2500.066.7019962
CGCTCCC451.19413926E-464.444996
TCTACTA1600.063.4380345
Produced by FastQC (version 0.11.2)