FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pr8_a2_rr.351000000a9511.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pr8_a2_rr.351000000a9511.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933499
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG132690.6862687800717766No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA84070.43480756907554646No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG70600.365141124976015No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT40950.21179219642730615No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC40580.20987856730207774No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA40210.20796493817684933No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG37990.19648316342547886No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA36570.1891389651610888No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT35280.182467123075833No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT34690.179415660416685No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT34450.17817438747059086No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT34370.17776062982189283No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA34130.17651935687579876No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT32610.1686579615505361No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA31480.16281363476267635No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG29370.15190077677826572No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG29320.15164217824782947No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT28890.14941823088607753No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT27810.14383250262865407No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC27360.14150511585472764No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA26200.13550562994860613No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC24790.12821315139030326No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC24720.1278511134476925No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT23990.12407557490332294No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT23290.12045519547721514No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT22010.11383507309804661No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGCTCGCTGCCTTTTCGTC21320.11026641337802605No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT21000.10861138278323393No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA20360.10530132159364966No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT20050.10369801070494478No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG19970.10328425305624674No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT19900.10292221511363595No Hit
GGCTACGGCAGGTCCATACACACAGGCAGGCAGGCAGGACTTGTGAGCAA19550.10111202540058206No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT19390.10028451010318598No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGCC100.0070893206145.014025
GAGCGAA8200.0141.488081
ATTAGTA1550.0126.3123251
ACGGCCC256.466631E-6115.999214145
GCAAAGC203.8651217E-4108.760513
GCAAAAG64050.0103.807853
AGCAGGC3750.098.604428
AATACTA1950.092.95772
AGCAAAA73400.090.683152
ACTAATA1150.088.264848
GCAGGGT43950.087.5932549
TAACTAA259.3883363E-486.999405145
GCAGGGG16100.086.008749
AGCAGGG64700.081.0198758
TTAGTAG2450.079.9056852
CGTACCT851.8189894E-1276.7721255
TACTATT2400.075.528144
AGCGAAA15400.075.3319552
AAGCAGG103950.075.118817
GAGCAAA91300.073.862811
Produced by FastQC (version 0.11.2)