FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pr8-vww_5-1_rr.351000000a9a50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pr8-vww_5-1_rr.351000000a9a50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156198
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG59530.5148772096128864No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA32960.2850722800074036No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG30400.26293074369614894No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG27120.2345619002973539No Hit
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA20130.17410512732248284No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA17830.15421234079284No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA17410.15057974499177476No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG17020.14720662031935705No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA16770.14504436091396108No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT16500.14270912075613346No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT16310.14106580360803253No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC15980.13821162119290986No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT15840.13700075592588812No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA15790.13656830404480894No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG15570.1346655157680605No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT14600.12627594927512414No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC14180.12264335347405894No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT13920.12039460369244716No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA13300.11503220036706516No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT13180.11399431585247509No Hit
ACCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGT12380.10707508575520802No Hit
CTGAAAGAAGAATCCGATGAGGCACTTAAAATGACCATGGCCTCTGTACC11900.10292354769684778No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT11850.10249109581576858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG202.130817E-6144.999081
GAGCGAA4150.0143.252121
GCAAAAG29200.089.386873
GCAGGGT21450.081.118379
AGCAGGG29700.077.385028
GAGCCCC300.001935867172.49954145
GCAGGGG8050.069.347399
CAAAAGC37950.069.159344
AAGCAGG49650.064.982267
AGCGAAA9400.064.0183262
GTAACAA7550.063.3798264
ACGTGCA350.003566122162.1451532
AGCAAAA42850.061.4200022
GAGCAAA43900.061.1044041
GGATAGC2400.057.3979575
TTCGGAT2950.056.52507145
GCGGAGC651.0937927E-555.7688831
CAGGTCA2000.054.374669
AGGGTGT6600.053.8277476
CGGAGCA553.2186246E-452.729222
Produced by FastQC (version 0.11.2)