FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pr8-vww_100-1_rr.351000000a9a36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pr8-vww_100-1_rr.351000000a9a36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453373
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG97520.6709908605705486No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA59830.4116630761683339No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG50340.34636669320263963No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG38450.26455699947639044No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT29050.19987986566421695No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA28540.19637078712759903No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG28320.19485706697454816No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT27590.1898342682848794No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA26960.18549952421023372No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC26340.18123358559709035No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT26330.18116478013558807No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG26250.18061433644356956No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA25320.17421542852385452No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA23890.16437624752902386No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT23470.16148641814592674No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT23140.15921583791635044No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC22790.15680764676376951No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT21760.14972068422903137No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT21290.14648682753842268No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA21000.14449146915485564No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA20790.14304655446330708No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT19660.1352715373135458No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC18180.1250883290112036No Hit
ACCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGT17990.12378102524265966No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC17900.12316177608913885No Hit
CTGAAAGAAGAATCCGATGAGGCACTTAAAATGACCATGGCCTCTGTACC16770.11538675893937758No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG16050.1104327657112111No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT15720.1081621854816348No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT15580.10719890902060243No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGCTCGCTGCCTTTTCGTC15420.10609802163656544No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT15190.10451549602201224No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT15130.10410266325299837No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG14990.10313938679196599No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA14990.10313938679196599No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT14850.10217611033093363No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT14840.1021073048694313No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA100.007089818145.009382
GCGGGGT100.0070912763144.999429
GAGCGAA8000.0140.482681
GCAAAAG43850.0101.357763
GCAGGGT31800.090.0546659
AGCGAAA13250.084.816822
AGCAAAA53250.083.873982
ATTAGTA1400.082.8653641
GCAGGGG9550.081.98929
AGCAGGG45050.081.2704858
CAAAAGC60200.074.067864
AAGCAGG75500.073.459977
GAGCAAA64950.069.3255841
TCTACTA851.6916601E-1068.2373665
AGCAGGC2450.068.060958
GAGCCCC502.0105892E-457.999763145
CTATAGG400.00605070954.3766484
TTAGTAG2150.053.9569852
AATACTA2150.053.9569852
AAGGGTG10100.053.1204265
Produced by FastQC (version 0.11.2)