FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pr8-vww_10-1_rr.351000000a9a43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pr8-vww_10-1_rr.351000000a9a43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445798
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG83070.5745615915916331No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA47930.3315124242805703No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG42040.2907736765440262No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG34120.23599423985923343No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA26860.18577975623150675No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC24560.16987158648718562No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG24100.16668995253832142No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA23400.16184833565961498No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA23330.16136417397174432No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT23110.1598425229527223No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT23060.15949669317567183No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT22270.15403258269827458No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG20950.14490267658414246No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA20560.14220520432314887No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT20320.14054522139330666No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT19890.13757108531067272No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC19560.1352886087821397No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA18850.13037782594802316No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT18820.1301703280817929No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT18230.12608953671259748No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT16240.11232551158598919No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC15840.10955887336958552No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA15810.10935137550335523No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC14950.10340310333808733No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT14550.10063646512168366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4950.0134.754651
AGAAGCG800.099.689365
GCAAAAG39650.096.912273
GCGGAGC406.116916E-790.629821
GCAGGGT26900.086.243979
CGGAGCA353.4374785E-582.858682
AGCAGGG38800.081.283738
CGTACCT451.2330293E-680.557055
GCAGGGG10350.079.853799
GAGCAGA1100.079.0951161
AGCAAAA51950.073.966732
AAGCAGG63600.070.905417
CAAAAGC55200.070.005844
GAGCAAA57400.068.335521
AGCAGGC2950.066.357168
AGCGAAA10300.064.7584842
GAAGCGG1250.063.801196
GAGCCCC702.3355278E-762.141865145
TCTACTA851.3056706E-859.7069975
TTCGGAT3350.056.267757145
Produced by FastQC (version 0.11.2)