FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pr8-vww_1-5_rr.351000000a9a79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pr8-vww_1-5_rr.351000000a9a79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635992
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA74700.456603699773593No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC37520.22934097477249277No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG31650.19346060371933357No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT29770.18196910498339847No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC23280.14229898434711175No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC21310.13025736067169033No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA19240.11760448706350643No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT19030.11632086220470517No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT18920.1156484872786664No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT18850.11522061232573265No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT17140.10476823847549377No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA16840.10293448867720624No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT16530.10103961388564248No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG100.007088867145.016452
GGAGCGT100.007089515145.012013
GAGCGAA1150.0132.414411
AGGATTG11650.0108.288925
AGCACGC1100.0105.472961
CGGTGCA5750.0104.664042
GCACGCA1200.096.6776352
CACGCAC1300.094.815553
GAGCAGA2000.094.266461
GTAACAA17950.094.1135564
ACGCACT1350.091.301074
GCGGAGC1750.091.158771
CGCACTT1350.085.930425
GAAACAC11550.080.350524
GGATTGA15550.079.26466
AACACGT755.638867E-1177.337376
CGGAGCA1700.076.7734152
ACGTGCA809.822543E-1172.5082242
AAACACG13050.070.559245
AGCATCT9400.070.1898355
Produced by FastQC (version 0.11.2)