FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pr8-vww_1-1_rr.351000000a9a6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pr8-vww_1-1_rr.351000000a9a6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047643
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA39940.38123673808730646No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG30140.28769342228220873No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA17300.16513258810491743No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG16620.15864182741640043No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC16150.1541555663522784No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG15060.14375125877803793No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT14070.13430147483446175No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG11900.11358831204904725No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC10690.10203857611800965No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA100.007090003145.00623
TTCGGAC100.007091015144.99928145
GAGCGAA2400.0126.886481
GAGCAGA1200.0108.759841
CACGCAC404.814865E-9108.754653
AAGGTCG301.5995343E-596.666195
GCACGCA502.2673703E-887.007872
AGCACGC502.2673703E-887.007871
GTAACAA10000.086.2745744
GGTCGTT353.437437E-582.8567357
ACGCACT651.5643309E-978.076544
CGCACTT651.5643309E-978.076545
CGGTGCA3300.072.506562
GCAAAAG18600.068.215293
ACGTGCA651.3972385E-766.929142
AACACGT554.061858E-665.908776
GCAGGGT12650.063.6162879
CGTTTTC1150.063.0431677
AGCAGGG17450.062.3206068
TTAGGAA350.003566620262.142554
Produced by FastQC (version 0.11.2)