FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pr8-vww_1-10_rr.351000000a9a86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pr8-vww_1-10_rr.351000000a9a86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274834
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA72770.570819416488735No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG39540.31015802841781753No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC30850.24199229076099318No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT25620.20096734163036126No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC20840.16347226383984112No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC18880.14809771311402112No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT17350.13609615055764124No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA17010.13342913665622347No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT16140.12660471873200746No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT15400.12080004141715706No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT15340.12032939190514216No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAACAAAAACTGAAAATCAA14940.11719172849170952No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA14750.115701338370329No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT13890.1089553620314488No Hit
ATCACATTTTTCGTTACTACAACCCCTTTCAACGTATAGTCAGATGCATT13770.10801406300741902No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT12850.10079743715652391No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG100.007089593145.010241
CACGCAC750.0135.34293
GCACGCA750.0135.34292
AGCACGC750.0135.34291
GAGCGAA750.0125.6755451
ACGCACT900.0112.7857364
AGGATTG10300.0111.2214365
CGGTGCA4500.0111.174512
CGCACTT1000.0101.5071645
GTAACAA16050.093.059544
GGATTGA12250.090.557426
AGCATCT8100.086.827125
GAAACAC8100.085.036874
CGGAGCA1500.082.172472
GAGCAGA2300.081.96231
ACGTGCA755.638867E-1177.33882
GCGGAGC1800.076.533181
GAGCATC8250.075.581094
TAGAAAC11700.070.646012
AAACACG10000.068.879865
Produced by FastQC (version 0.11.2)