FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pr8-vww_1-100_rr.351000000a9a93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pr8-vww_1-100_rr.351000000a9a93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675012
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA82300.49133976353602243No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC42670.25474444362189647No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT33920.20250601189722822No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG32920.19653590541440896No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC26900.16059586438783724No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC26350.15731230582228664No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT22750.13581992248413743No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT21670.12937220748269265No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA21140.12620805104679847No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT20620.12310359567573248No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA20240.12083495521226116No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT18570.11086487738595305No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT18410.10990966034870198No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18350.10955145395973283No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAACAAAAACTGAAAATCAA18140.10829773159834077No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17940.10710371030177694No Hit
GGCCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA17320.10340224428242901No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAG100.0070907976145.003343
AGGATTG9300.0124.730345
AGCACGC650.0111.547691
CGGTGCA6050.0111.4488452
GAAACAC8800.0110.396974
GTAACAA21400.0103.667534
TTCGGAA352.762681E-7103.570724145
GAGCAGA2200.0102.167541
CGGAGCA1850.094.056222
CACGCAC800.090.627093
ACGTGCA1150.088.26292
TAGAAAC12350.088.0587162
GGATTGA12950.086.7754756
GAGCATC8150.086.287754
GCACGCA850.085.296082
AGCATCT9650.084.14455
GCGGAGC2500.084.1069641
ACGCACT1000.079.749464
CGCACTT950.076.315275
ACAAGAG29950.074.072957
Produced by FastQC (version 0.11.2)