FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_pa-259459_rr.351000000a9abc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_pa-259459_rr.351000000a9abc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571681
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA65920.2563303924553628No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC53720.20889060501671866No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT41640.16191743843812664No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC35880.13951963715561924No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT35500.13804200443212047No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA32590.1267264485758537No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC31240.12147696390026601No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC27940.10864489024882946No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC27880.10841157981880334No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCACGCGATGGCAG26680.10374537121828097No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC26560.10327875035822873No Hit
CCCCTGTACCACATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC25860.10055679534125732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGTC502.3062876E-672.505211
CTAACGG406.6647306E-572.500984
CGAGTTT2950.071.2721564
TCGAGTT3250.064.694443
TTCGAGT3250.064.694442
TCTTCCG9750.057.2571835
TAGACGG400.006052104854.374676145
CGCGTCC400.006052104854.374676145
GGCTTCT10500.053.861011
TTCCGTG10700.052.1736037
GTTCGAG4250.049.4741441
CTTCCGT11650.048.541436
CAACTCC1801.8189894E-1244.305294145
CAGTTCG1851.8189894E-1243.1086965
ACAGTTC1901.2914825E-1038.1584134
TAACCAC1001.3919467E-436.250497
TATAGGA18750.035.574512
ATATAGG19550.034.861841
GCGAAAG4800.034.739389
GTTTGAA6250.033.6404577
Produced by FastQC (version 0.11.2)