FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_p6-740_rr.351000000a9aa0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_p6-740_rr.351000000a9aa0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058125
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTAGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAA39210.19051320983905254No Hit
ATGTTGGATGAGGGGGTAGAACCAGATGACGTCGATTGCTGGTGCAACAC38350.18633464925599758No Hit
CTTAATACATTCACCAACCTGGTGGTGCAGCTCATTCGGAACATGGAGGC30980.15052535681749163No Hit
GTATAGAGTGATGACTCGCAGACTGCTAGGTTCAACACAAGTTGGAGTGG30660.14897054357728515No Hit
CTCTATACACTCCATCTGTGGTCTCCCCCTTTTTTACTTCCTTGGGAGCA25290.12287883389006984No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGGGCTTGGTTTCC23590.11461888855147281No Hit
TACATATACTCATCTCCAGGTTTGTTGGGATTCCTGCCTATGCGCCCCCT23060.11204372912238081No Hit
ACTCTATATCAACAGTTGGCTTGTCCTGTGCCATTACGGTAACACAACCT21450.10422107500759187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA100.0070915036144.99931145
CCGTAAG301.10199835E-7120.835692
GTTCGAG1550.065.4883351
TTCGAGT1450.065.0012742
TGACGGG350.003567106662.142567145
CAGTTCG753.7626705E-758.0011334
TTACACC1007.203198E-1058.0011334
TCTTCCG6850.055.0375755
GCGTCAG400.0060501654.3787081
AGTTGCC400.00605190554.374744145
TCGAGTT1750.053.85823
TAATGCC1353.6379788E-1253.7047584
CCCGTAA701.6959339E-551.789241
CTTCCGT7450.050.605026
CTTACAC1152.4865585E-950.435773
AGTTCGA901.324086E-648.334285
ATAGTCG604.945833E-448.334286
CGTCAGA450.009639828548.334282
GTGTAAG951.9209801E-645.7925951
CTCTTAC1351.0262738E-842.9658931
Produced by FastQC (version 0.11.2)