FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_ny238_plasmid_rr.351000000a9b92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_ny238_plasmid_rr.351000000a9b92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202035
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG57200.25975972225691235No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG54830.2489969505480158No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA49590.22520078018741754No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC44740.20317569884220735No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG41760.18964276226308846No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT36070.16380302765396554No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT34250.1555379455821547No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA31620.14359444786299946No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT31370.14245913439159685No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT27190.12347669314974558No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC26960.1224322047560552No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG26180.11889002672527912No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT25630.11639233708819342No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT24710.11221438351343188No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT24490.1112153076585976No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT24200.10989834403177061No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA24030.10912633087121686No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG22850.10376765128619664No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT22400.10172408703767197No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC22300.10126996164911094No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA22220.10090666133826211No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT22060.10018006071656445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACA1950.063.2072224
CAGCGTC350.003566603662.144922
CAGGGGT350.003566603662.144922
ACGCTCC400.006052141554.374332145
GATGTAT5600.051.786261
CCAGAGT1152.4865585E-950.4364553
TACTGAT17650.049.293427
GAGTGGC1203.623427E-948.3349386
CCTAACC450.00964112948.332745145
CGACGCC1056.9123416E-848.33274145
AGAGTGG1255.2004907E-946.401545
TAGGACC3450.044.1298949
GATGATA3400.042.646538145
CGGACAT3100.039.7593845
ATACATC10100.038.7636643
TCCGGCC750.001483862938.666195145
TATACTG31350.038.3904275
TTCGGAC3700.037.2309653
AGTGGCC1604.5925844E-836.25127
TTATACT6250.035.9611934
Produced by FastQC (version 0.11.2)