FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_mr766a_rr.351000000a9b42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_mr766a_rr.351000000a9b42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484716
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG56480.2273096804624754No Hit
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA49230.19813129548809602No Hit
GAATGGTTTTGGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCA45190.18187189199892462No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGAGCTTGGTTTCC36930.1486286561522524No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA34250.13784271522379216No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA32220.12967276743096595No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG30050.1209393749627724No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA28760.1157476347397449No Hit
CCCACAAGGTGGAGGTCGCTGCTGTGATCAGAGCTCCAGCCTCCCCCCAT28030.1128096732181867No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT26870.10814113162228602No Hit
ATTCCACACCATGAGCATGTCCTCAGTGGTCATCCATTCTCCCTTTCCAT25220.10150053366259966No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC25170.10129930342139704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT100.0070892145.015673
CGGTATC100.0070904796145.006934
CTAACGT203.8650777E-4108.761762
TACGTGA502.2693712E-887.004165
TCGAGTC2650.084.8204963
CGAGTCT2650.084.815374
CGACAGT1050.082.8661041
TTCGAGT2750.081.736112
GTTCGAG2850.081.412311
ACAGTTC1150.075.6603553
TAACGTA300.001935251872.5078353
ACTAACG300.001935251872.5078351
CAGTTCG1200.072.5034564
ACGTGAT608.0339305E-872.5034566
GCGACAG651.397857E-766.930311
GAGTCTG3650.063.5646825
TTAGAGG350.003566451662.1458244
TAAGACA350.003566451662.1458244
TCTTCCG9300.060.0297435
TCCGGGG851.3065801E-859.705128145
Produced by FastQC (version 0.11.2)