FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_mex-i-44_rr.351000000a9b0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_mex-i-44_rr.351000000a9b0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746083
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA75550.27511914242941676No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC63700.231966768666497No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC44710.16281372412996986No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC43650.15895368056974243No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT43290.15764272237947652No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC42210.15370984780867875No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC39640.14435106295039152No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC38960.14187480859100032No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC37710.1373228704303548No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT34650.1261797258130945No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC33600.12235609775815225No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA33120.12060815350446437No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG32260.11747642004994023No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC30840.11230541829944689No Hit
ATAATATACAGATTGTAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA29300.10669743048553157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA1050.0110.4820562
CGAATAA1050.0110.478035
GTCTAAT1550.0107.584391
ACGAATA1100.0105.456294
CTAACGA1150.0100.873081
CTAATAT1700.098.093433
TAATATT1800.096.6682744
GGCTTCT8700.084.16961
CGGACAC703.092282E-1182.858525
CGAGTTT3200.079.298194
TCGAGTT3400.074.636313
TCGTTAG502.3065877E-672.5038453
TTCGTTA502.3065877E-672.5038452
TTCGAGT3500.072.5038452
GTTCGAG3500.072.502521
CGTTAGA502.307088E-672.50124
TAAAATA300.001936115872.499886145
CGGATCG300.001936115872.499886145
TTTCCGT2950.068.8147056
TTTTCCG2950.068.8147055
Produced by FastQC (version 0.11.2)