FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_mex-2-81_rr.351000000a9af0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_mex-2-81_rr.351000000a9af0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365759
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA74010.3128382899526114No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC59510.251547177882447No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC48220.20382464993264318No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC47250.19972448588381148No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC42700.18049175761351854No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT37220.15732794422424262No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT32740.13839110408118493No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC31380.13264242046632815No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC29860.12621742113207643No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG29690.12549883568021933No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC29630.12524521728544624No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC29300.12385031611419423No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG27860.11776347463963996No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC27530.11636857346838796No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA27090.11450870524005191No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAA26560.11226840941955626No Hit
GCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGT25960.10973222547182532No Hit
GTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGA25760.10888683082258169No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA24980.10558979169053145No Hit
GTCATATACTTGGTCATGATACTGCTGATTGCCCCAGCATACAGCATCAG24510.10360311426480888No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTTC950.0106.845894
GCGTCAG259.383082E-487.0122761
GAGTAAA259.388599E-486.999405145
GCAGGGG259.388599E-486.999405145
TCGAGTT3450.086.16433
CGAGTTT3550.083.735364
GTTCGAG3600.082.58111
TTCGAGT3700.080.3423842
AGATGTT300.001936122872.499504145
ACAGGTC554.059915E-665.918391
TTGTTAA554.059915E-665.918391
CAGTTCG1550.065.48625
CAACTCC902.8194336E-1064.444145
TTCTTCG350.003566307262.1463662
TTAAGCC702.3353459E-762.1450544
CGGTTTT350.003567203262.142426145
TGTAGGG350.003567203262.142426145
GTTAAGC606.8061763E-660.4200823
AGTTTGA5000.059.4521066
GTTTGAA5100.058.285157
Produced by FastQC (version 0.11.2)