FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_lp_neg_ctrl_rr.351000000a9baf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_lp_neg_ctrl_rr.351000000a9baf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4072
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTCATCTCCAGGTTTGTTGGGATTCCTGCCTATGCGCCCCCT210.5157170923379175No Hit
CCTCTGGGCAGCGCTGGCATGTGTGACAGGCATGGGTCCAGCCAGAATGA140.343811394891945No Hit
CATATACTCATCTCCAGGTTTGTTGGGATTCCTGCCTATGCGCCCCCTCC120.29469548133595286No Hit
TCCATACATATACTCATCTCCAGGTTTGTTGGGATTCCTGCCTATGCGCC110.27013752455795675No Hit
GTCTTCATCAGTCTCTGCGCACCCACCTCCATACATATACTCATCTCCAG100.2455795677799607No Hit
TCTCTGAGATGTCAGTTGTTACGACGAAGTCCCACTCTTGATTTTTTGTT80.19646365422396855No Hit
CCATACATATACTCATCTCCAGGTTTGTTGGGATTCCTGCCTATGCGCCC80.19646365422396855No Hit
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA70.1719056974459725No Hit
CCTCCATACATATACTCATCTCCAGGTTTGTTGGGATTCCTGCCTATGCG70.1719056974459725No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT60.14734774066797643No Hit
GTGTAGGTTATTCCGGCAGATGCAACCTGATAGGCCAGCCAAACAGGAAG50.12278978388998035No Hit
CTTCTATCTGTGTAGGTTATTCCGGCAGATGCAACCTGATAGGCCAGCCA50.12278978388998035No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG50.12278978388998035No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT50.12278978388998035No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCTC151.14221155E-4145.05
ATGTGCC100.006834025145.07
ATAGATG100.006834025145.03
AGATGTG100.006834025145.05
CAGAAAT151.14221155E-4145.02
TAGATGT100.006834025145.04
TCATAGA100.006834025145.01
GTGCCCG100.006834025145.09
GAAATCT258.7214494E-487.04
AGAAATC258.7214494E-487.03
GCAGAAA258.7214494E-487.01
AATCTCG350.00331550262.1428576
ATCTCGT400.005626545854.3757
TCTCGTA400.005626545854.3758
CTCGTAT450.00896555348.3333329
AAAAAAA2200.009114046.59090930-34
Produced by FastQC (version 0.11.2)