FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_ibh-30656_rr.351000000a9ad6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_ibh-30656_rr.351000000a9ad6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3200544
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTCGTTA46550.1454440245158323No Hit
ATTCATTAGAGTGTGACCCAGCCGTCATAGGAACAGCTGTCAAGGGAAAG43300.13528950078486657No Hit
CTAATGAATAGTCCTCTCTGACCTTCAGCCAAACACTGGTGTGGAAGACC37170.11613650679384503No Hit
TATTTACACAGTGACAAGAAATGCCGGTCTGGTTAAGAGACGTGGAGGTG36950.11544912364897968No Hit
TCATAGTACTGGTGTATGGGCACAGCACCTTTATGCAGAAGGCCCCTGGT34040.10635691932371497No Hit
ATTCCACACCATGAGCATGTCCTCATTAGTCATCCATTCTCCCTTTCCAT33150.1035761420558505No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAATCAAGT32740.10229510983132868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTATC100.0070901127145.010071
TTCGAGT4500.0101.50232
CGAGTTT4600.0100.870274
TCGAGTT4650.099.78723
GTTCGAG4650.098.2326351
GCGACAG850.093.830051
CTAAAAT651.6370905E-1189.232793
GCGTCAG353.4372497E-582.862911
CGAGGGG554.3977707E-879.09023145
AGCAGGG554.3977707E-879.09023145
CGACAGT1000.072.501652
AACTTCG406.665195E-572.500516
TAAAATT406.665195E-572.500514
ACAGTTC1050.069.04814
TCTATCC3450.067.24588
CACGCCC451.1943089E-464.44389145
AGTTGCC451.1943089E-464.44389145
ATCTTTT350.003567342262.14232145
GCTTTTT7950.062.0139852
GGCTTTT8150.061.3846361