Basic Statistics
Measure | Value |
---|---|
Filename | HW23FBCXY_l01n01_s1_ibh-30656_rr.351000000a9ad6.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3200544 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTCGTTA | 4655 | 0.1454440245158323 | No Hit |
ATTCATTAGAGTGTGACCCAGCCGTCATAGGAACAGCTGTCAAGGGAAAG | 4330 | 0.13528950078486657 | No Hit |
CTAATGAATAGTCCTCTCTGACCTTCAGCCAAACACTGGTGTGGAAGACC | 3717 | 0.11613650679384503 | No Hit |
TATTTACACAGTGACAAGAAATGCCGGTCTGGTTAAGAGACGTGGAGGTG | 3695 | 0.11544912364897968 | No Hit |
TCATAGTACTGGTGTATGGGCACAGCACCTTTATGCAGAAGGCCCCTGGT | 3404 | 0.10635691932371497 | No Hit |
ATTCCACACCATGAGCATGTCCTCATTAGTCATCCATTCTCCCTTTCCAT | 3315 | 0.1035761420558505 | No Hit |
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAATCAAGT | 3274 | 0.10229510983132868 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTGTATC | 10 | 0.0070901127 | 145.01007 | 1 |
TTCGAGT | 450 | 0.0 | 101.5023 | 2 |
CGAGTTT | 460 | 0.0 | 100.87027 | 4 |
TCGAGTT | 465 | 0.0 | 99.7872 | 3 |
GTTCGAG | 465 | 0.0 | 98.232635 | 1 |
GCGACAG | 85 | 0.0 | 93.83005 | 1 |
CTAAAAT | 65 | 1.6370905E-11 | 89.23279 | 3 |
GCGTCAG | 35 | 3.4372497E-5 | 82.86291 | 1 |
CGAGGGG | 55 | 4.3977707E-8 | 79.09023 | 145 |
AGCAGGG | 55 | 4.3977707E-8 | 79.09023 | 145 |
CGACAGT | 100 | 0.0 | 72.50165 | 2 |
AACTTCG | 40 | 6.665195E-5 | 72.50051 | 6 |
TAAAATT | 40 | 6.665195E-5 | 72.50051 | 4 |
ACAGTTC | 105 | 0.0 | 69.0481 | 4 |
TCTATCC | 345 | 0.0 | 67.2458 | 8 |
CACGCCC | 45 | 1.1943089E-4 | 64.44389 | 145 |
AGTTGCC | 45 | 1.1943089E-4 | 64.44389 | 145 |
ATCTTTT | 35 | 0.0035673422 | 62.14232 | 145 |
GCTTTTT | 795 | 0.0 | 62.013985 | 2 |
GGCTTTT | 815 | 0.0 | 61.384636 | 1 |