FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_hrv16_rr.351000000a9b78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_hrv16_rr.351000000a9b78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911787
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACC2551.8189894E-1234.11934
GAGGGTC850.00274120834.1192974
TACCGAG4353.6379788E-1223.3331837
CGGGTAC4850.022.4249953
GGTACCG4358.367351E-1121.6677175
CCCGGGT4409.822543E-1121.4238431
CCGAGCT4251.4697434E-920.4704579
TACTAGG2857.0904916E-620.3529782
GTCGTAG2155.050277E-420.2357581
CCTAGCA1800.00430694520.138769
ACCTAGC1800.00430694520.138768
ATACTAG5103.274181E-1119.9040163
TTACTAG2959.547659E-619.6641251
CATAGGG2659.341122E-519.1529222
CTAGTTC2751.2415857E-418.4554396
GTACCGA4959.746145E-917.5766096
TATAATA2500.001392303317.4017962
CCGGGTA6303.6379788E-1117.2636872
ATACACG3804.379599E-617.1718834
CCTATAG2550.001589748717.0605853
Produced by FastQC (version 0.11.2)