FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_hrv14_rr.351000000a9b6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_hrv14_rr.351000000a9b6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951977
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACT2600.041.828092
CTCCTAC2950.039.32381
TATAGGG13250.024.0757373
GGGAGTG14150.022.5440357
GTATATG30000.021.9926531
TATATAC26850.021.3317053
GTATATA25950.020.3959061
CCTAAGG2505.9790553E-520.3002223
ATATACA33300.018.941654
ATACAAT15700.018.9333296
TATACAA15450.018.7704325
CCCTAAG2400.001060179118.1255052
GAGGTCC7700.017.8895039
GTATTAG5301.1823431E-917.7838171
ATATGAC25200.017.5497953
TATATAG2901.8667718E-417.500195
GTCATAT20000.017.400781
GGGTACC4256.737355E-717.0590114
CATATAC29600.016.9005243
TAGAGTG19800.016.4774535
Produced by FastQC (version 0.11.2)