FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_hb-2016-1840_rr.351000000a9b1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_hb-2016-1840_rr.351000000a9b1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457409
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA71800.29217765540860313No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC58890.23964264800853258No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC45470.185032284003192No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT41750.16989438876475182No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT34920.14210088756084152No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC34660.1410428626248215No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC33210.13514233894317146No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC31470.12806171052519136No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC30620.12460278284974133No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG30340.12346337138018132No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC29910.1217135609090713No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC28720.11687106216344124No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA27270.11097053848179118No Hit
ATAATATACAGATTATAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA25360.10319812452872111No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAGT300.001935667872.503883
CAGTTCG1650.070.308231
GGCTTCT8700.066.67161
TCGAGTT3350.064.928853
CAACTCC902.8194336E-1064.44396145
CGAGTTT3400.063.9714134
TTCGAGT3450.063.0468562
GGTCCGA350.003566076562.1474571
GTTCGAG3500.062.147451
GTCCGAC350.003566363862.146192
CCCGATC350.00356693962.143664
TCTTCCG9700.059.7945865
CTTCCGT9950.057.5635576
ATACTCG400.006050160654.379021
ATTGTAG400.006050647754.3779142
TTCCCGA400.006050647754.3779142
TTCCGTG10700.053.527647
GTTCACC450.00963798148.3369061
GATTGTA450.00963798148.3369061
GCGTCAG450.00963798148.3369061
Produced by FastQC (version 0.11.2)