FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_fss-13025_rr.351000000a9ac9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_fss-13025_rr.351000000a9ac9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687641
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAA66700.24817302608495703No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC60450.22491843218644159No Hit
GTATATAACACTTATTCATCCCCATTGTGGTTGGAAAAGATATGGCCTCC43210.16077296037677652No Hit
CCCCTGTACCGCATCTCGTTTCCTTCTTTGAGAAGTCCACCGAGCACCCC40730.15154553751784558No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCTTGGAGGGACAGGTAC39920.14853174214859796No Hit
ATATAGGACATGGGCTTACCATGGAAGCTACGAGGCCCCCACACAAGGGT39050.1452947026779246No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG39020.14518308062721175No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT34700.12910950532455787No Hit
ATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAACC32170.1196960457144388No Hit
ATGCTGGATGAGGGGGTAGAACCAGATGACGTCGATTGTTGGTGCAACAC32060.11928676486182492No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA31980.11898910605992391No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGGGCTTGGTTTCC31420.11690549444661694No Hit
CTTAATACATTTACCAACCTAGTGGTGCAGCTCATTCGGAATATGGAGGC29230.10875708474457713No Hit
ATAATATACAGATTATAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA27900.10380850716297303No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACA203.865738E-4108.757331
TCGAGTT3050.092.712893
CGAGTTT3050.092.709444
AACTAGC554.1654857E-1092.277234
GCGTCAG406.1178434E-790.631111
ATGTTCG1100.079.096241
TCGAGTG1150.075.655865
ACATACT2150.074.189674
CGTCAGA502.3063785E-672.504892
ACAGGTC702.8139766E-972.504881
CGCTCTG300.001935724372.503545
TGTTCGA1250.069.604682
TTCGAGT4150.068.137122
ACAGTTC1009.094947E-1265.253194
CGAGTGG1350.064.4475866
GTTCGAG4450.063.5436061
GTGCAGG350.00356594462.14823
CAGTTCG1051.6370905E-1162.1458855
CCCTCGC350.003566469862.145885
CTACTAT350.003566469862.145885
Produced by FastQC (version 0.11.2)