FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_extraction_neg_ctrl_rr.351000000a9a1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_extraction_neg_ctrl_rr.351000000a9a1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47050
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGCGCAGGCGTCGCTGCACCTGCCGCTGTGTTCGC1210.25717321997874604No Hit
ATCTGCAACAGATCAAAGAAACAAAAGTGAAAACATGGAAGCCAGGGATG960.20403825717321997No Hit
TTACAGAGCAAGTCAAAACCAACTGGGACCCAACAAGAAAAAAACAGAAT950.20191285866099895No Hit
CTGTAGGGTGGAGTCTGAATTCCAAGAATTGAGTTCCCCATCTGTTAAGC850.1806588735387885No Hit
ATATATGTGTATGTAACTGGATGATGTCCTCTGGTCACAGTTTTACTTTC850.1806588735387885No Hit
GTATGTAACTGGATGATGTCCTCTGGTCACAGTTTTACTTTCTTTTTTTT770.1636556854410202No Hit
GTAGAAACACGAGCACGAACACGAGCACGAACACGAATGCGAATGCGAAT750.1594048884165781No Hit
GTTGCAGATGGTAGTGTTTGTTAACTCTGTCTGGTTTCCTGGTTTCATTG680.14452709883103082No Hit
GTACGAAGTGCTTCATTTCCTGTAAACTAGTTAATACATTGGCTGTGGTG670.14240170031880978No Hit
GTCTTATGCAGGAATTTGGAGGTGACAGTAATAGCAGGATTACCGTAAAC640.13602550478214664No Hit
ACCCTACAGACACCAAATTAGTCACAGGTAGGAATATTATAGATGTTAAC620.13177470775770456No Hit
ATTTCATACTTAACATGAAGTATCTCAACGCTTTACAGAGCAAGTCAAAA600.1275239107332625No Hit
GTATTAACTAGTTTACAGGAAATGAAGCACTTCGTACTGTGCATTTGCAT590.12539851222104145No Hit
ACCGTAAACCTTGCAAACATAAGGATTATCAAGACAATTTCTTCTGACTC580.1232731137088204No Hit
GTCAAAGAACAGATATCGAACTACATAGAAAGTGAAACTGTGGGCCGGGC570.12114771519659936No Hit
GTATAGAGGGTGGCAGGTACCCAGTAGTAACTGTAGAAGGGAAATAGACT560.11902231668437832No Hit
ATAATATTCCTACCTGTGACTAATTTGGTGTCTGTAGGGTGGAGTCTGAA550.11689691817215729No Hit
GTTTACGGTAATCCTGCTATTACTGTCACCTCCAAATTCCTGCATAAGAC540.11477151965993623No Hit
CTCCATACTCCATCCATCCCTGGCTTCCATGTTTTCACTTTTGTTTCTTT520.11052072263549415No Hit
CTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAGAGACACTA520.11052072263549415No Hit
GGATATGAGAGTCCTGGACGACAGAATCCCGTGGAGTCTTGATCTCTCAT510.10839532412327312No Hit
CTCTAAAATGGCTCAACACTGAAATCTTGAGGATGAAAAACAGAATTTGC510.10839532412327312No Hit
GTCATATAGGATATGAGAGTCCTGGACGACAGAATCCCGTGGAGTCTTGA510.10839532412327312No Hit
AGTTAATACATTGGCTGTGGTGATGACTATGGGAACACTGGGGATTTTTT500.10626992561105207No Hit
TGTTAACTCTGTCTGGTTTCCTGGTTTCATTGGGGACTTGGTGATTCACT500.10626992561105207No Hit
CTCTATGACAGAAATGTCACGTCCTTCTTTTCTCCACCTTCTCCTTTCCC490.10414452709883104No Hit
CCTTAAAAACTTGTTTTCTCTATGACAGAAATGTCACGTCCTTCTTTTCT490.10414452709883104No Hit
ACATAGAAAGTGAAACTGTGGGCCGGGCACGGTAGCTCATGCCTGTAATC490.10414452709883104No Hit
TCTTAATTCAGGGCAGTCCTCTGTGTCAATGCTGGATGCAAATGCACAGT480.10201912858660998No Hit
GCATAAGACAATCAATTTTGGGGGGAAAAAATCCCCAGTGTTCCCATAGT480.10201912858660998No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCTA203.8273726E-4108.86096
TGAACCT203.8273726E-4108.86095
GAAGCGG259.2930795E-487.088721
AAGGGTC259.332478E-486.996188
AACCTAA259.332478E-486.996187
AAAGGGT259.332478E-486.996187
GTAAGGA300.001916479272.573941
AGTAACA1150.063.107773
CTATAAC350.003531110262.206233
TTACTGA350.003531110262.206231
TATAACA350.003531110262.206234
GCTATAA350.003531110262.206232
CTGAACC350.003531110262.206234
GAAAGGG350.003531110262.206236
TAAGGAA350.003531110262.206232
CCTAAAG350.003546038962.1401259
TAATTAT651.0711177E-555.8261071
TAACAAG1301.8189894E-1255.8261075
ACCTAAA400.006016297354.3726128
GTAACAA1353.6379788E-1253.758474
Produced by FastQC (version 0.11.2)