FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_dakar-41671_rr.351000000a9b35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_dakar-41671_rr.351000000a9b35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013037
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGTTTTGGCGATACTAGCCTTCTTGAGATTCACAGCAATCAAACCA47910.23799860608622694No Hit
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTTGCCA30980.15389682355565248No Hit
ATTCCACACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTTCCAT30170.14987305250723162No Hit
CCATAAGTCCTCCCTTTTTCCCAAATAGGGAATGTCTGTCCATTTTGTTA25130.12483625487261288No Hit
GATATATATCAACAAGGGTTGAAATGGGCGAGGCGGCTGCTATCTTCATG24510.1217563313540685No Hit
CATCAATAGATGGGGTTCCGTGGGGAAGAAGGAGGCTATGGAAATAATAA23440.11644097947529032No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA22800.11326170358518No Hit
GGCTTTTTCCGTGCCATGGCGTTATCGGCCTGACTATGAGCTTGGTTTCC21920.10889019923627832No Hit
CTATAAGGGATCCACACCATAAGTCCTCCCTTTTTCCCAAATAGGGAATG21150.10506513293098935No Hit
CTCTATTCCACACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTT21050.10456837107315961No Hit
GTATATAACTTTTTGGCTCGTCGAGCTTCCCAGGAGCCAGGCTATAGCTA20790.10327679024280229No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATTC100.007090952145.0035
AGGTGTA100.007090952145.0032
GTATCAC100.0070914784144.99942145
AGTCGCG151.2302095E-4144.9994145
GCGACAG600.0120.841831
AGTCGCC352.7628448E-7103.57101145
CGGTTCC259.388292E-486.99964145
GCGTCAG353.4377703E-582.8588644
GTTCGAG3000.079.755621
GCCTGTT651.5643309E-978.078543
TTCGAGT3200.074.767172
GTCAGAT406.66414E-572.50156
TAAGGTT300.001935869372.50156
CGTCAGA406.66414E-572.50155
CGAGTTT3400.070.369114
CAGGGAT554.0613995E-665.913731
CGACAGT1150.063.0479161
CGTTCAG350.003566734762.1441462
CCCTTTA606.807257E-660.417922
CAGTTCG1251.8189894E-1258.0011984
Produced by FastQC (version 0.11.2)